Prof. Dr. Ulrich F. Keyser
Cavendish Laboratory, University of Cambridge, UK
Donnerstag, 04. Februar 2021, 16:00 Uhr
Polymer and ion dynamics in nanopores revealed by designed DNA nanostructures
DNA nanotechnology is transformative for experiments that require molecular control over the shape of nanometer-sized objects. In combination with nanopores DNA self-assembly allows for novel experiments that reveal the physics of ions, and polymers on the single molecule level .
Nanopore sensing, best known for DNA sequencing, translates the three-dimensional structure of molecules into ionic current signals. Designed DNA molecules enable multiplexed protein sensing with an all-electrical approach  and may pave the way to data storage applications . Here, I will discuss our recent developments to detect and localise structures as accurately as possible along DNA molecules approaching super-resolution microscopy [3,4]. The localisation is enabled by the signal-to-noise ratio and asymmetry of our glass nanopores . In the second part of the talk, I will discuss how we can study ion dynamics using DNA origami structures [6,7] including the creation of an all-optical voltage sensor based on Foerster resonant energy transfer . The talk will end with our efforts to actuate DNA origami by incorporation of PNIPAM for temperature activated motion  and an outlook on future development and questions.
 U. F. Keyser. Enhancing nanopore sensing with DNA nanotechnology. Nature Nanotechnology, 11:106-108, 2016.
 N. A. W. Bell and U. F. Keyser. Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores. Nature Nanotechnology, 11:645-651, 2016.
 K. Chen, J. Kong, J. Zhu, N. Ermann, P. Predki, and U. F. Keyser. Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores. Nano Letters, 19(2):1210-1215, 2019
 K. Chen, F. Gularek, B. Liu, E. Weinhold, and U. F. Keyser. Electrical DNA Sequence Mapping Using Oligodeoxynuc leotide Labels and Nanopores. ACS nano (published online) 2021. https://dx.doi.org/10.1021/acsnano.0c07947?ref=pdf
 N. A. W. Bell, K. Chen, S. Ghosal, M. Ricci, and U. F. Keyser. Asymmetric dynamics of DNA entering and exiting a strongly confining nanopore. Nature Communications, 8:380, 2017.
 V. V. Wang, N. Ermann, and U. F. Keyser. Current enhancement in solid-state nanopores depends on three-dimensional DNA structure. Nano Letters, 19(8):5661-5666, 2019.
 C.-Y. Li, E. A. Hemmig, J. Kong, J. Yoo, S. Hernández-Ainsa, U. F. Keyser, and A. Aksimentiev. Ionic Conductivity, Structural Deformation and Programmable Anisotropy of DNA Origami in Electric Field. ACS nano, 9(2):1420-1433, 2015.
 E. A. Hemmig, C. Fitzgerald, C. Maffeo, L. Hecker, S. E. Ochmann, A. Aksimentiev, P. Tinnefeld, and U. F. Keyser. Optical voltage sensing using DNA origami. Nano Letters, 18(3):1962-1971, 2018.
 V. Turek, R. Chikkaraddy, S. Cormier, B. Stockham, T. Ding, U. F. Keyser, and J. J. Baumberg. Thermo-responsive Actuation of a DNA Origami Flexor. Adv. Funct. Mater., 28(25):1706410, 2018