DNA Electrophoresis

From ICPWiki
Revision as of 19:38, 5 September 2011 by Wmueller (talk | contribs)
Jump to: navigation, search
End labeled free-solution electrophoresis (ELFSE): Simulation snapshot

Electrophoresis is one of the main techniques to separate DNA molecules by size and has shown its effectiveness in the sequencing of entire genomes, including our own. This success story also increased the demand for improved and faster sequencing methods, in order to meet the upcoming challenges.

Over the last years, many experimental investigations have been performed in the field of electrophoresis, in order to improve existing methods or to develop new separation techniques which yield a faster or better separation. These experimental improvements gave rise to many interesting questions that still need to be dealt with from a theoretical point of view.

The aim of this research is to provide these fundamental understandings of the electrophoretic separation process by means of computer simulations using ESPResSo.

Special topics that are currently under investigation are:

  • free-solution electrophoresis
  • end-labeled free-solution electrophoresis
  • electrophoresis in confined geometries

Hydrodynamic interactions are implemented in simulations by using a frictional coupling of the MD particles to a Lattice Boltzmann fluid. This efficient way of treating hydrodynamics enables a detailed study of the complex electrohydrodynamic interactions.

Coworkers

  • Christian Holm: Project supervisor
  • Kai Grass: PhD thesis on Hydrodynamic effects in polyelectrolyte electrophoresis

Collaborators

  • M. Hervé Cottet, CNRS laboratory, Université Montpellier 2, Montpellier, France
  • Ulrich Scheler, Leibnitz-Institute for Polymer Research (IPF), Dresden, Germany
  • Gary W. Slater, University of Ottawa, Ottawa, Canada

Publications

Hydrodynamics with Lattice Boltzmann fluid:


Links

Elfse.png